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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
Article No 3
September -2009 / Volume -1 / Issue - 7/ Article No -3 / Research Article
IN VITRO ANTIBIOTIC ACTIVITY OF ISOLATED VOLATILE OIL OF CITRUS SINENSIS
September -2009 / Volume -1 / Issue - 7/ Article No -3 / Research Article
IN VITRO ANTIBIOTIC ACTIVITY OF ISOLATED VOLATILE OIL OF CITRUS SINENSIS
Mr.Singh Amandeep
1S.B.S college of pharmacy, patti.,Punjab,India
E-mail: amandeep.676@rediffmail.com
E-mail: amandeep.676@rediffmail.com
SINGH AMANDEEP1*, AHMED R. BILAL1.
ANIL DEVGUN1.
ANIL DEVGUN1.
ABSTRACT
The main aim of this study is that due to increasing concerns about the development of antimicrobial resistance amongst pathogenic bacteria, alternative strategies have been sought that do not use antibiotics to reduce pathogenic bacteria from foods and patients. A natural compound that has potent antimicrobial properties is citrus peel, which contains a variety of essential oils that inhibit the growth of or kill pathogenic bacteria. Further the objective of study is to verifying a possible antimicrobial activity of citrus oil. For this we had isolated the volatile oil from fresh peel of citrus sinensis collected from the market. Antibacterial activity of oil was evaluated by paper disc diffusion method against Bacillus subtilis and Escherichia coli using erythromycin as a standard and DMSO as a control sample. Zone of inhibition of E.coli and B. subtilis was 13mm and 17mm respectively when compared with the results of standard i.e. 31mm against E.coli. and 32mm against B.subtilis.Therefore in the future several citrus based phytoconstituents may be used in treating certain life threating diseases.
KEYWORDS: Citrus oil, Paper disc diffusion method, Erythromycin, Zone of inhibition.
The main aim of this study is that due to increasing concerns about the development of antimicrobial resistance amongst pathogenic bacteria, alternative strategies have been sought that do not use antibiotics to reduce pathogenic bacteria from foods and patients. A natural compound that has potent antimicrobial properties is citrus peel, which contains a variety of essential oils that inhibit the growth of or kill pathogenic bacteria. Further the objective of study is to verifying a possible antimicrobial activity of citrus oil. For this we had isolated the volatile oil from fresh peel of citrus sinensis collected from the market. Antibacterial activity of oil was evaluated by paper disc diffusion method against Bacillus subtilis and Escherichia coli using erythromycin as a standard and DMSO as a control sample. Zone of inhibition of E.coli and B. subtilis was 13mm and 17mm respectively when compared with the results of standard i.e. 31mm against E.coli. and 32mm against B.subtilis.Therefore in the future several citrus based phytoconstituents may be used in treating certain life threating diseases.
KEYWORDS: Citrus oil, Paper disc diffusion method, Erythromycin, Zone of inhibition.
